Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

FP5

IMPECABLE Résumé de rapport

Project ID: G5RD-CT-2000-00372
Financé au titre de: FP5-GROWTH
Pays: Bulgaria

Development of bioluminescence and optical detectors for medical and biological applications

The sensitivity of the photomultipliers with improved cathodes was investigated for detection of the biological luminescence. New results on tissue luminescence including improvements of the original detector system were obtained. The advance in sensitivity was considerable (ie>10,000 times).

Medical luminescence exploitation of the PM tubes was presented with new examples of biologically relevant signals and spectral response, particularly for the detection of cancer.

Three cationic zinc phthalocyanines (ZnPcs), tetrakis-(3-methylpyridyloxy), tetrakis-(3-hexyl-pyridyloxy), and tetrakis-(3-dodecylpyridyloxy) phthalocyanine zinc (ZnPcMe, ZnPcXe and ZnPcDo) have been studied as advanced fluorescent contrast agents for pigmented melanoma. UV-Vis spectroscopic properties of the monomers were investigated. Their photophysical behaviour as a substantial part of dye-induced fluorescence was evaluated. The selective accumulation and labelling capacity towards B16F0 pigmented melanoma tumor were determined. Melanin containing cells were isolated and incubated with ZnPcs at several time intervals (1, 2.5 and 6hours) following the kinetics of cellular uptake. A highest accumulation was found for ZnPcXe. A comparable uptake was detected for the extremely lipophilic ZnPcDo and hydrophilic ZnPcMe. Fluorescence diagnostic potential of ZnPcs towards pigmented melanoma by using an argon-dye laser detection set-up was demonstrated.

Contact

Latchezar AVRAMOV, (President)
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Fax: +359-2-9745742
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