Community Research and Development Information Service - CORDIS

Development of model challenge systems to assess the pathogenic potential of suspected larval pathogens, and to assess adverse effects of candidate probionts

A general limitation for testing the effectiveness of probiotic bacteria in in vivo trials is to have a reliable and reproducible challenge model with a pathogenic strain. For Roseobacter 27-4 a challenge model using the fish pathogen Vibrio anguillarum 90-11-287 has been developed.

The pathogenic strain was selected due to the antagonism showed in vitro by Roseobacter 27-4 against V. anguillarum 90-11-287 and V. splendidus. V. anguillarum showed different pathogenicity depending on the administration mode.

When added to the larvae via rotifer, the mortality obtained in 10 d old larvae was 28-44% with respect to mortalities in unchallenged larvae, which was adequate for testing the probiotic strain. This challenge model could be used to test the in vivo probiotic activity of other strains.

Reported by

CSIC
Instituto de Investigaciones Marinas, Eduardo Cabello 6
36208 Vigo
Spain
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