Community Research and Development Information Service - CORDIS

Development of a chemostat system for larval turbot rearing

The lack of control over the microbiota in the larval rearing of marine fish is one of the main factors affecting variability of growth and, particularly survival in cultures Unpredictability of changes in the microflora of larval rearing can be diminished or avoided in axenic systems. Rearings in axenic chemostat systems have the advantage of their flexibility from the point of view of manipulation of the microflora and therefore the study of interactions between specific bacteria.

A procedure has been developed for the larval rearing of turbot larvae in axenic chemostats. The rearing of axenic turbot larvae larval rearings in chemostats required the use of axenic microalgae, rotifers and newly hatched turbot larvae. For that, two protocols were proposed for the production of axenic rotifers and larvae, respectively. Protocols were based on baths of the organisms in mixtures of antibiotics, from which, mixture IIM resulted more appropriate.

One of the advantages of sterile chemostats is that both microalgae and rotifers grow faster than in non-axenic systems. By applying the protocols proposed, turbot larvae could be successfully reared under axenic conditions until day 10 using initial densities of 105 cells of Isochrysis ml{-1}, 1 rotifer ml{-1} and 25 larvae l{-1}. Performances achieved with the methodology proposed were adequate for experimental purposes. Survivals at day 10 were about 40%, with a maximum of 80%.

Reported by

CSIC
Instituto de Investigaciones Marinas, Eduardo Cabello 6
36208 Vigo
Spain
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