Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

Secondary genetic alterations in MCL

The main objective to characterize secondary genetic alterations on three different independent levels: DNA, RNA and protein, has been successfully carried out. Combination of these different approaches and the exchange of material and results between the network partners have contributed extensively to identifying the critical master genes in the malignant transformation and progression of MCL.

One group has used two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) to analyse total cellular protein extracts to identify qualitatively differentially expressed proteins before and after treatment with 5-aza-dC. Using a high-density cDNA microarray, 128 primary tumours including 56 MCL cases, small lymphocytic lymphoma (31) and marginal zone lymphoma (37) have been analysed.

Several clusters of genes involved in distinct biological functions such as cell-cycle control, cell differentiation, and intracellular signalling, significantly discriminated the three histological subtypes. Different groups succeeded in substantially narrowing down the critically deleted region in 1p and 13q. Although up to now the tumour suppressor genes in 1p and 13q have not been identified, a number of candidate genes have been studied for down regulation and mutations. Our comprehensive approach has given important new insights into genes and proteins that trigger the malignant transformation and progression in MCL.

Reported by

Hannover Medical School
Carl-Neuberg-Str. 1
30625 Hannover
Germany
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