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Antibodies against human and mouse proteins

Aim: to raise polyclonal antibodies in rabbits against human CFTR and CF-related (mouse) proteins for use on antibody chips, in immunocytochemistry and in Western blots.

- Rabitt poyclonal antibodies against human CFTR
- CFTR: Extracellular loops
- EnaC: Extracellular loops of a,b, and g subunits

- Rabitt polyclonal against Cytokeratins
- CK8 (Cytokeratin 8)
- CK18 (Cytokeratin 18)

- Rabitt polyclonal against human beta defensins 1,2,3,4
- Chicken polyclonal against human beta defensins 1,2,3,4
- Mouse monoclonals against huamn beta defensins 2,3,4

Mouse proteins:
- Mouse chloride channels CIC-Ka and CIC-Kb (see for selection discriminating peptides: option 3 from e-mail correspondence with Ann Andries, 18/09/03)

- Mouse barttin (=regulator of CIC-K channels, identified in mouse nasal epithelium)

- Mouse a-, b-, and g-subunits of the epithelial sodium channel ENaC

- Mouse b2-adrenergic receptor (b2-AR)

- The CFTR-regulated chloride/bicarbona te exchangers;
-- 0 mouse SLC 26 A3 (=DRA, CLD)
-- 0 mouse SLC 26 A6 (=PAT-1)
-- 0 mouse SLC 26 A9 (lung-specific)

- Mouse PDZ-adapter protein CAP-70 (binds to C-terminus of CFTR and CLD).

- Mouse CFTR chloride channel
-- 0 first extracellular loop (cf. sequence hCFTR, Chemicon Ab).
-- 0 C-terminal13 aa or cloned C-terminal fragment (80aa).

- Mouse ROMK (CFTR-regulated epithelial potassium channel in kidney and lung).

- Mouse chloride channel CIC-3.

- Mouse SGK-1 (serum-and glucocorticoid-activated protein kinase, regulates ENaC).

- Mouse chloride channel CIC-2.

- Mouse YAP-65 (=Yes-associated protein, involved in CFTR-ENaC coupling).

- Mouse D-AKAP2 (AKAP and putative CFTR regulator).

Reported by

University of Leuven
Krakenstraat
3000 Leuven
Belgium
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