Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

FP5

SLRV-C Résumé de rapport

Project ID: QLK2-CT-2001-70356
Financé au titre de: FP5-LIFE QUALITY
Pays: Netherlands

Knowledge on the availability of monocytes in blood and expertise with methods to obtain these monocytes

Knowledge of the availability of monocytes in blood:
Monocytes are the only peripheral blood cells carrying the SRLV’s -genetic material and it is estimated that this is the case in only 0.1% of these cells. Hence it is of crucial importance to know if these cells, which form a very small part (literature: 0-4%) of the white blood cell fraction, are available in any given blood sample. Studies in goats and sheep of different ages, gender, health and SRLV-infection status demonstrated that every 8ml blood contains a sufficient number of monocytes to allow reliable testing. This means that blood is a reliable (and a practical) substrate for testing.

Expertise with methods to obtain these monocytes:
In view of the low percentage of monocytes carrying the viral genes it is important to obtain all the monocytes from the blood sample in the highest possible concentration. Several options have been compared for efficiency and practicality. It appeared that the best results were obtained by differential centrifugation, yielding a fraction containing lymphocytes and monocytes (PBMC’s) followed by separation of the monocytes using their property to engulf particles that can be trapped in a magnetic field. The efficacy of different particles and engulfing conditions was compared, leading to an optimum monocytes collection protocol, which can be maintained as the gold standard method.

Reported by

University of Utrecht
3508 Utrecht
Netherlands