Servizio Comunitario di Informazione in materia di Ricerca e Sviluppo - CORDIS

FP5

HEALTHYPIGUT Sintesi della relazione

Project ID: QLK5-CT-2000-00522
Finanziato nell'ambito di: FP5-LIFE QUALITY
Paese: Italy

Lactobacillus amylovorus: resident porcine gastrointestinal microbiota exerts protection for enterotoxigenic E. coli (ETEC) strain K88 in porcine intestinal IPEC-1

Bacterial therapy for man and farmhouse animals is largely based on the concept that specific strains selected from the commensal gut microbiota are involved in resistance to intestinal diseases. The underlying mechanisms, however, are poorly understood.

Here the possible protective effects of two phylogenetically closely related Lactobacillus species, against intestinal damages promoted by enterotoxigenic Escherichia coli (ETEC) strain K88 were examined in vitro. Using fully differentiated swine intestinal cell line IPEC-1, antibacterial activity was measured by assessing the number of the adhering ETEC cells after an inoculation with porcine isolate Lactobacillus amylovorus-like or with a non-porcine strain of L. amylovorus DSMZ.

The results revealed that ETEC adhesion was reduced by L. amylovorus-like, but not L. amylovorus DSMZ. Upon L. amylovorus-like treatment, the distribution of tight junctional and cytoskeletal proteins were preserved from ETEC induced damages.

To investigate whether these effects were mediated by cytokines and were associated with prevention of inflammation, and whether this was counteracted by the two L. amylovorus strains, the expression of pro- and anti-inflammatory cytokines was analysed. ETEC-induced up regulation of interleukin (IL)-1b and IL-8 was significantly diminished when the cells were treated with L. amylovorus-like, but not with L. amylovorus DSMZ. Together, the data suggests that L. amylovorus-like exerts a protective effect against ETEC adhesion and invasivity and against the membrane barrier disruption promoted by ETEC, possibly via blocking IL-1b and IL-8 expression in IPEC-1 cell line.

Contatto

Elena MENGHERI, (Head of Unit)
Tel.: +39-06-51494523
Fax: +39-06-51494550
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