Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

List of 10 genes evaluated and confirmed as LE-markers for traits related to meat quality and carcass traits

A short list of functional candidate genes covering 52 loci was established. Screening for polymorphism was done by comparative sequencing of a set of DNAs of animals of the breeds Duroc, Pietrain, and German Landrace shared by all partners. In summary in 36 out of 49 genes screened for polymorphism either SNPs or InDels were detected that are suitable for genotyping. PCR-RFLPs, PCR-SSCPs, single base extension assays, TaqMan assays, as well as melting curve analysis protocols were established for high throughput genotyping of the polymorphisms. First selective genotyping was performed for 22 loci. Chi-square analyses as well as analyses of variance indicated possible association with drip loss of a number of candidate genes.

The most promising twelve loci were finally selected to perform the association analysis using the whole set of sample and data. Pordictor has reported evidence for 10 genetic markers in linkage equilibrium (LE-marker) that are significantly associated with several pork quality traits within population.

A short summary of perspectives for each gene:
- ANK1: there are some significant genetic effects for driploss and associated traits in Duroc crossbreds, but gene effects are neither clearly additive nor dominant.

- CA3: there are some major gene effects across populations, especially for conductivity, cookloss and intramuscular fat. This seems an interesting gene for further research.

- EPOR: there are some significant genetic effects for several traits across populations, especially for driploss and associated traits (including pH) but gene effects are not consistent for traits across populations and most of them are neither clearly additive nor dominant. Further research for this gene is relevant for driploss related traits.

- MYOP: there are some major gene effects across populations for pH and loin depth/weight. This seems an interesting gene for further research and perhaps implementation in breeding programs for both traits.

- bR10D1: there are some significant genetic effects for several traits, but gene effects are not clearly additive nor dominant, partly due to the distribution of the alleles.

- PDGFRA: the gene with least interesting results.

- HGMA2: this gene has only significant effects in 1 population.

- ELKS: there are some major gene effects across populations for colour and lean weight. This seems an interesting gene for further research and perhaps implementation in breeding programs for both traits.

- NME1: there are some significant genetic effects for several traits, but gene effects are neither clearly additive nor dominant, partly due to the distribution of the alleles.

- TTN: there are some significant genetic effects for several traits, but gene effects are neither clearly additive nor dominant.

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18196 Dummerstorf
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