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FP5

AEEC INFECTIONS Sintesi della relazione

Project ID: QLK2-CT-2000-00600
Finanziato nell'ambito di: FP5-LIFE QUALITY
Paese: Hungary

Acquisition of phages encoded toxins (Stx1 and Stx2) by AEEC strains

Objectives:
- To examine the putative role of EPEC for the evolution of EHEC by acquisition of phages encoding Stx;
- To establish an in vivo phage transduction model for wild-type E. coli (EPEC) strains.

Major finding during the project:
Establishing an in vivo transduction model. In porcine ligated intestinal loops we repeatedly were able to lysogenise a porcine EPEC strain 1390 of O45 with stx2::cat phage induced from an O157 donor strain. On the other hand the human prototype strain E2348/69 was not lysogenised with stx2::cat phage under the same conditions.
Chromosomal integration site of F3538(stx2::cat). F3538(stx2::cat) occupies a new so far un-described site for Stx-phages in the chromosome of E. coli O157:H7 strain 3538 and tranductants.

Stability of F3538(stx2::cat) in the lysogens. F3538(stx2::cat) could not be mobilised from the transductant neither in vitro nor in vivo.

In summary, it was demonstrated that EHEC can be developed from EPEC by acquisition of stx phage (stx2) in vivo using a porcine EPEC strain in a porcine ligated intestinal loop system.

Contatto

Bela NAGY
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Fax: +36-12521069
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