Community Research and Development Information Service - CORDIS

FP5

ENDOPORATOR Report Summary

Project ID: IST-2001-33141
Funded under: FP5-IST
Country: Switzerland

Electric field modelling

Analytical and numerical simulations of electric field and current distribution in the tissues during electroporation have been performed. The model was has been used to optimise the design of electroporation electrode. We have shown that tissue electroporation requires relatively large electrodes. In the case on co-planar interdigitated electrodes, the depth of electroporation is comparable to the spacing between electrodes. The field concentration at the edge of electrodes is the cause of superstimulation and subsequent possible tissue damage. Needle tips can help to penetrate the tissue. An electrode-electrolyte model has been developed and measurements have shown its predictive value. This model has then been further extended to take into account a tissue, which is modelled as a network of finite RC-elements. To verify the models an "artificial tissue" based on the hydrogel pHEMA has been developed. Our tissue model has been applied to determine the best electroporation pulse signal. We anticipate improvement of electroporation efficiency and reduction of cell damage when replacing usual pulsed signal with a modulated signal at a frequency that is related to relaxation effects in tissues.

Reported by

EPFL
ILMIS4 IMM EPFL
1015 Lausanne
Switzerland
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