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  • An optimised method for determining the presence of the seedborne fungus Alternaria alternata, a potential producer of harmful mycotoxins, on material of carrot (Daucus carota)

An optimised method for determining the presence of the seedborne fungus Alternaria alternata, a potential producer of harmful mycotoxins, on material of carrot (Daucus carota)

The method is based on a blotter test and involves examination of the fungal morphology after 7 days of incubation. For testing seeds a deep-freeze step to prevent germination is necessary. The diagnosis involves microscopic observation of the fungus sporulation on incubated carrot seeds, seedlings and roots. The test is completed within 7 days which is advantageous in terms of work planning in a laboratory. Based on the results from a ring test with 5 laboratories and 4 levels of seed contamination, it was concluded that the deep-freezing-blotter-test provides reliable information on the seed contamination with Alternaria alternata.

Reported by

AUGUST CIESZKOWSKI AGRICULTURAL UNIVERSITY IN POZNAN
Baranowo, Szamotulska 28
62-081 Przezmierowo
Poland
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