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FP5

ACE Sintesi della relazione

Project ID: EVK1-CT-2001-00100
Finanziato nell'ambito di: FP5-EESD
Paese: United Kingdom

Estrogenic mixture effects at low concentrations

A question of considerable relevance for the regulation of chemicals and their mixtures is whether combination effects occur when each chemicals is present at low effect concentrations. This issue was addressed experimentally by using a variety of in vitro assays for estrogenicicty, including estrogen receptor binding, the yeast estrogen screen, the E-Screen and the ER-CALUX assay. "Low effect concentration" was operationalised in terms of concentrations equal to EC01 or no-observed effect levels. By combining sufficiently large numbers of estrogenic chemicals it was possible to demonstrate that mixture effects occurred even when each individual component was present at levels around EC1 or NOEC. In the E-Screen assay it was not possible to estimate EC01 for many chemicals because their concentration-response relationships exhibited a very shallow slope in the low dose range, levelling off at the 1% effect level. In these cases, EC2.5 were used.

Reported by

THE SCHOOL OF PHARMACY, UNIVERSITY OF LONDON
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