Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

Structure of the GDNF receptor GFR1 and its GDNF binding surface

We have solved the 1.8 Å crystal structure of the GFR1 domain 3 and discovered a new protein fold. It is an all-alpha five-helix bundle stabilized by five disulfide bridges. The structure was used to model the homologous domain 2, the other half of the GDNF-binding fragment, and to construct the first structural model of the GDNF-GFR?1 interaction. Using site-directed mutagenesis, we identified closely spaced residues, Phe213, Arg224, Arg225 and Ile229, comprising a putative GDNF binding surface.