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Overexpression of GDNF in the intact nigrostriatal dopamine system

Overexpression of GDNF in the intact nigrostriatal dopamine system results in downregulation of tyrosine hydroxylase (TH), a rate limiting enzyme in dopamine synthesis. However, autoradiography binding to dopamine receptors (D1 and D2), and dopamine transporter (DAT) in the striatum, show no significant change indicating that the reduction in TH does not result in any marked decrease in dopamine levels. We also show that the loss of TH is not a result of loss of dopaminergic cells in the substantia nigra since there is a normal number of dopamine neurons as determined by markers independent of TH. This effect was not seen by applying Artemin, another GDNF family ligand in the same paradigm.

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LUND UNIVERSITY
Sölvegatan 17
221 84 LUND
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