Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

Development of modified tests for detecting virus neutralising antibody to SAV and infectious SAV in sera

Prior to the project, antibodies to SAV in sera were detected using a virus neutralisation (VN) assay, which required approximately 7 days to complete, which involved relatively large reagent volumes and which required input from a person skilled in reading SAV-induced cytopathic effect. During the project Partner 1 developed a modified virus neutralisation test which could be completed in 3 days, which used a microtitre plate-based format involving very small reagent volumes, and which used immunoperoxidase (IPX) staining to detect the presence of non-neutralised virus. The immunostaining depended on the availability of SAV-specific monoclonal antibodies which were generated prior to the project and which are owned by Intervet International. The development and evaluation of the modified VN test showed that some sera contained high levels of infectious virus. This observation led to the development of a method for detecting viraemic serum samples, which is run in parallel with the modified VN test. Application of the viraemia detection assay has resulted in the isolation of many SAVs in cell culture. The application of these 2 methods involving the detection of infectious virus and virus-specific antibody has proved to be of considerable use in diagnosing SAV infections and this approach is likely to be adopted by other Partners and laboratories outside the Consortium.

Reported by

Queen's University of Belfast
Veterinary Sciences Division, Stormont
BT4 3SD Belfast
United Kingdom