Community Research and Development Information Service - CORDIS

Production of collection of SAV cell culture isolates

Prior to the project approximately 15 cell culture isolates of salmonid alphavirus (SAV) had been obtained mainly by laboratories of Partners 1, 3 and 4. These had been obtained from clinical tissue specimens from outbreaks of PD and SD in Ireland, Norway and France. One of the sub-objectives of the project was to expand the number of cell culture isolates to facilitate investigation of biological (antigenic and pathogenic) and genetic diversity. This objective was achieved through efforts made by Partners 1, 3 and 4. Most of the new isolates were made with serum samples through the application of the IPX-based method for detecting viraemia in serum samples. Work with the greater range of SAV cell culture isolates has shown the existence of 3 genetic subtypes, which appear to have a geographical occurrence. In addition, investigations have shown that representative isolates display a relatively high level of antigenic similarity as determined by monoclonal antibody reactivity, and that representative PD virus isolates from Norway and Ireland exhibit similar pathogenicities in experimental infection studies. Although some work to characterise the new isolates has been undertaken, much work remains to be done and it is possible that some of the isolates made within the project may have distinctive characteristics that may make them useful commercially eg as low pathogenicity virus strains that may be suitable as live attenuated virus vaccines.

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Contact

David GRAHAM, (Veterinary Research Officer)
Tel.: +44-2890525749
Fax: +44-2890525787
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