Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

Development of immunohistochemical test for detecting SAV in acutely affected pancreas

Immunohistochemistry (IHC) is used for detection of antigens in sections made for histology. The method, as used in diagnostics of fish diseases, is usually not very sensitive. In diseased fish that suffer from infectious diseases, however, the etiologic agent is usually present in large numbers. The benefit of the IHC is that it may detect the etiologic agent in tissue lesions that are characteristic for the disease and thus it provides a fairly strong indication of a connection between the agent and the characteristic lesion(s).

In the present project an infection experiment delivered tissues from PD infected and from uninfected (control) fish that were used as a basis for the development of the IHC for detection of SAV. The IHC was subsequently used on field samples, both those collected for the project and samples submitted for disease diagnostics outside the project.

The method works well on pancreatic samples from acute PD-diseased fish. The IHC is thus very useful in distinguishing acute PD from acute IPN as these two different viruses give quite similar pancreatic lesions when they cause disease in salmonid fishes.

This IHC did not detect SAV in PD-characteristic lesions in heart and muscle.

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Torunn TAKSDAL, (Research scientist)
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