Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

FP5

BLUETONGUE VACCINATI Berichtzusammenfassung

Project ID: QLK2-CT-2001-01722
Gefördert unter: FP5-LIFE QUALITY
Land: United Kingdom

A comprehensive database o BTV sequences from isolates across the world has been established to enable molecular epidemiological studies

A reference collection of BTV isolates has been established at IAH-Pirbright. Details of the viruses are available on the website:
www.iah.bbsrc.ac.uk/dsRNA_virus_proteins/ReoID/viruses-at-iah.htm

Vaccine strains of BTV 1, 2, 9 and 16 originally generated at OVI plus a Turkish vaccine strain are also in the collection. Sequences for genome segments 2, and 6 of these viruses have been determined see: www.iah.bbsrc.ac.uk/dsRNA_virus_proteins/btv_sequences.htm

Sequence comparisons of genome segments 2 from multiple strains of BTV 1, 2, 4, 9 and 16 have been completed and phylogenetic trees, which indicate the likely geographic origins of the European BTV strains are shown on a project website at:
www.iah.bbsrc.ac.uk/dsRNA_virus_proteins/orbivirus-phylogenetic-trees.htm

The databases also contain sequence analyses of S 10 for many Greek BTV isolates showing how they group into clusters. In addition, sequencing studies of genome segments 2 and 6 from strains of all 24 serotypes of BTV provide a database to support typing of all BTV isolates by sequence comparisons alone. Using such information primers have been designed to distinguish between genome segments from the live attenuated vaccines and European field strains and is listed at:
www.iah.bbsrc.ac.uk/dsRNA_virus_proteins/ReoID/rt-pcr-primers.htm

These databases will form a basis for future molecular epidemiological studies of BTV isolates and are freely available as an international resource.

Verwandte Informationen

Kontakt

Peter MERTENS, (Head of Arbovirus Research Group)
Tel.: +44-1483232441
Fax: +44-1483232448
E-Mail-Adresse
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