Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

SYBR-green RT-PCR Assay for porcine cytokine genes

A SYBR-green Assay was established for measuring IL-4, IL-2, IFN-g and IL-5, with the housekeeping genes Cyclophilin and GAPDH. It gave similar results to the multiplex RT-PCR, and was also appropriate to measure changes in mRNA-levels of cytokines upon stimulation of PBMCs with CSFV.

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