Servizio Comunitario di Informazione in materia di Ricerca e Sviluppo - CORDIS

Novel Platelet antigens (German team)

The German partner performed complete sequence analysis of P-selectin and its ligand PSGL-1 in healthy blood donors and patients with coronary heart disease (CHD). By using PCR-SSP they genotyped 261 CHD patients and 214 controls for 5 SNPs in P-selectin and 2 SNPs in PSGL-1. The P-selectin 715P allele was more frequent among CHD patients with
hypercholesterolemia compared to patients with normal cholesterol levels. A SNP (M62I) in the PSGL-1 gene was found close to the P-selectin binding site and the 62I allele revealed a higher prevalence in the control group indicating a protective effect of the mutation. In addition to the SNPs the PSGL-1 gene also revealed a variable number of tandem repeats (VNTR) polymorphism. The results of case/control studies showed that the PSGL-1 VNTR polymorphism is not a genetic risk factor for CHD. The P-selectin and PSGL-1 variants may represent the molecular basis of alloantigens.

More than 28,683 human genes were investigated by microarray analysis and in the receptor category 221 of 1,056 (20.9 %) analyzed genes were detectable. Among these, candidates were selected for further investigation.

The protein expression of IL-4R, IL-10R, IL12-R, IL-18R and CCR5 could be confirmed by flow cytometrical analysis of platelets. The interleukin, chemokine and cell adhesion receptors may be important for the role of platelets in inflammation. These membrane proteins may also carry novel alloantigens.

Informazioni correlate

Contatto

Harald KLUTER, (Professor, MD PhD - CR4 = German Team)
Tel.: +49-62-13706817
Fax: +49-62-13706818
E-mail