Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

FP5

RECOMBINANT BCG Informe resumido

Project ID: ICA4-CT-2000-30032
Financiado con arreglo a: FP5-INCO 2
País: Argentina

generation of rBCG-babesia antigen strains

Two recombinant BCG (rBCG) expressing the antigen RAP-1 from Babesia bovis. The rBCG contains expression vectors named pUS2001 and pMIP12 were a fragment comprising the rap-1 gene devoid of signal sequence was inserted. Both constructs have different promoters, ribosome binding sites and heterologous signal sequences. Western blot using specific anti RAP-1 monoclonal antibodies demonstrated the expression of rRAP-1 in BCG. Subcellular localization experiments indicated that rRAP-1. The construction in pMIP12 demonstrated to be stable in vitro and in vivo.

Reported by

NATIONAL INSTITUTE OF AGRICULTURAL RESEARCH
Los Reseros y Las Cabañas
1708 CASTELAR
Argentina
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