Servizio Comunitario di Informazione in materia di Ricerca e Sviluppo - CORDIS

FP5

RECOMBINANT BCG Sintesi della relazione

Project ID: ICA4-CT-2000-30032
Finanziato nell'ambito di: FP5-INCO 2
Paese: Argentina

generation of rBCG-babesia antigen strains

Two recombinant BCG (rBCG) expressing the antigen RAP-1 from Babesia bovis. The rBCG contains expression vectors named pUS2001 and pMIP12 were a fragment comprising the rap-1 gene devoid of signal sequence was inserted. Both constructs have different promoters, ribosome binding sites and heterologous signal sequences. Western blot using specific anti RAP-1 monoclonal antibodies demonstrated the expression of rRAP-1 in BCG. Subcellular localization experiments indicated that rRAP-1. The construction in pMIP12 demonstrated to be stable in vitro and in vivo.

Contatto

Ángel CATALDI, (Head of Unit)
Tel.: +54-11-46210199
Fax: +54-11-44812975
E-mail