Servizio Comunitario di Informazione in materia di Ricerca e Sviluppo - CORDIS

FP5

RECOMBINANT BCG Sintesi della relazione

Project ID: ICA4-CT-2000-30032
Finanziato nell'ambito di: FP5-INCO 2
Paese: United Kingdom

generation of leuD KO rBCG strains

I recombinant BCG strain was generated, rBCG-leuD, in which the leuD gene was deleted. The strain was therefore an auxotroph for the amino acid leucine. This allowed the use of plasmid vectors that provide the leuD gene in trans that complement the leuD mutation. This complementation allows the stable expression of foreign genes in BCG in the absence of antibiotic resistance markers.

Contatto

Johnjoe MCFADDEN, (Professor of Molecular Genetics)
Tel.: +44-1483686494
Fax: +44-1483300374
E-mail