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FP5

RECOMBINANT BCG Résumé de rapport

Project ID: ICA4-CT-2000-30032
Financé au titre de: FP5-INCO 2
Pays: Brazil

generation of rBCG strains expressing sm14 antigen

We have generated recombinant BCG strains that express the Schistosoma mansoni antigen, Sm14, in fusion with the beta-lactamase protein and signal sequence. Expression was obtained using a plasmid containing the upregulated beta-lactamase promoter, pBlaF. We have expressed Sm14 in BCG from the native gene. These rBCG-Sm14 strains induced a cellular Th1-type immune response, demonstrated by IFN-g production of cultured splenocytes upon stimulation with rSm14.

Reported by

Butantan
Butantan Institute
05503-900 Sao Paulo
Brazil