Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

FP5

RECOMBINANT BCG Berichtzusammenfassung

Project ID: ICA4-CT-2000-30032
Gefördert unter: FP5-INCO 2
Land: Brazil

generation of rBCG-anaplasma antigen strains

We have generated two recombinant Mycobacterium bovis BCG expressing the MSP1a antigen of Anaplasma marginale. The msp1a gene was amplified by PCR and cloned into the mycobacterial expression vectors pUS2000 and pMIP12. Stability of the vector was demosntrated in vitro and inside bovine cells. Expression of the recombinant antigen was confirmed by Western blotting using a monoclonal antibody against the Msp1 angigen.

Kontakt

Odir DELLAGOSTIN, (Head of Biotechnology Centre)
Tel.: +55-53-32757350
Fax: +55-53-32757551
E-Mail-Adresse
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