Community Research and Development Information Service - CORDIS

FP5

RECOMBINANT BCG Report Summary

Project ID: ICA4-CT-2000-30032
Funded under: FP5-INCO 2
Country: Brazil

generation of rBCG-anaplasma antigen strains

We have generated two recombinant Mycobacterium bovis BCG expressing the MSP1a antigen of Anaplasma marginale. The msp1a gene was amplified by PCR and cloned into the mycobacterial expression vectors pUS2000 and pMIP12. Stability of the vector was demosntrated in vitro and inside bovine cells. Expression of the recombinant antigen was confirmed by Western blotting using a monoclonal antibody against the Msp1 angigen.

Reported by

UNIVERSIDADE FEDERAL DE PELOTAS
Campus Universitario
96010-900 PELOTAS
Brazil
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