Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

FP5

RECOMBINANT BCG Informe resumido

Project ID: ICA4-CT-2000-30032
Financiado con arreglo a: FP5-INCO 2
País: Brazil

generation of rBCG-anaplasma antigen strains

We have generated two recombinant Mycobacterium bovis BCG expressing the MSP1a antigen of Anaplasma marginale. The msp1a gene was amplified by PCR and cloned into the mycobacterial expression vectors pUS2000 and pMIP12. Stability of the vector was demosntrated in vitro and inside bovine cells. Expression of the recombinant antigen was confirmed by Western blotting using a monoclonal antibody against the Msp1 angigen.

Reported by

UNIVERSIDADE FEDERAL DE PELOTAS
Campus Universitario
96010-900 PELOTAS
Brazil
See on map
Síganos en: RSS Facebook Twitter YouTube Gestionado por la Oficina de Publicaciones de la UE Arriba