Community Research and Development Information Service - CORDIS

Purified recombinant seabream PTHrP

Recombinant sea bream parathyroid hormone related protein was produced and purified using an E. coli system and one step purification process with continuous elution gel electrophoresis. The bioactivity of recombinant (1-125) sbPTHrP assessed using an in vitro scale bioassay was found to be equipotent to (1-34) PTHrP in stimulating cAMP accumulation. Assessment of the immunological reactivity of the isolated protein by Western blot revealed it cross reacted with antisera specific for the N-terminal and C-terminal region of PTHrP. In a radioimmunoassay specific for piscine N-terminal (1-34) PTHrP, the recombinant sbPTHrP (1-125) was equipotent with (1-34) PTHrP in displacing labelled 125I (1 34) PTHrP from the antisera. The recombinant sbPTHrP allows the development of region specific assays and to carry out studies aimed at defining post-secretory processing of this protein and its biological activity in fish.

Reported by

Centre of Marine Sciences
Campus de Gambelas
8005-139 Faro
Portugal
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