Community Research and Development Information Service - CORDIS

FP5

MIRACLE Report Summary

Project ID: EVK3-CT-2002-00087
Funded under: FP5-EESD
Country: Netherlands

Collection of environmental sequences

This collection of environmental sequences encompasses in total 434 sequences obtained from clone libraries. At this moment 14 clone libraries are still under construction and this work is in progress. In total 280 unique seciences were obtained.

From the North Sea 159 sequences of the 16S rRNA gene have been obtained of which 76 were unique and included 53 cyanobacterial sequences and 23 heterotrophic bacteria. From the Ionian Sea (Mediterranean) 129 sequences of the 16S rRNA gene were obtained of which 82 were unique, including 27 from cyanobacteria, 47 from heterotrophic bacteria and 8 from eukaryotic plastids. From the Baltic Sea 49 16S rRNA gene sequences were obtained, all of which were unique and included 42 cyanobacterial sequences and 7 of heterotrophic bacteria.

Further, 39 sequences were obtained for the phycoerythrin operon (cpeBA) from the Baltic Sea, all of which were unique and from cyanobacteria. A clone library of cDNA of transcripts of nifH (structural gene of nitrogenase, the N2-fixing enzyme) resulted in 39 sequences, all unique and belonging to cyanobacteria. The sequences will be submitted to GeneBank.

Related information

Reported by

NEDERLANDS INSTITUTE OF ECOLOGY
KORRINGAWEG, 7
4400AC YERSEKE
Netherlands
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