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MIRACLE Report Summary

Project ID: EVK3-CT-2002-00087
Funded under: FP5-EESD
Country: Netherlands

PCR primers for environmental clone libraries of Phototrophic Microorganisms

The following set of primers have been used for PCR of environmental DNA samples and for subsequent construction of clone libraries of phototrophic microorganisms. For the 16S rRNA gene and the intergenic transcribed spacer (ITS): Mir-PA8F and Cya23S-58R. For 16S rRNA gene: p16S 621cyanF and p16S 1438CyanR. For ITS-1: Mir-Bac1055F and PITS E Cyan R. For cpeBA either CyaCpe-F1 and CyaCpe-R1 or B3FW and SynA1R (M. Wood). For nifH a nested PCR with Yanni and nif 450 and nif down and nif up (J. Zehr). Primer sequences will be published in the primary literature.


Lucas Johannes STAL, (Head of Unit)
Tel.: +31-11-3577497
Fax: +31-11-3573616
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