Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS


BIOSAFE-VACCINE-VECT Berichtzusammenfassung

Project ID: QLK2-CT-2001-00874
Gefördert unter: FP5-LIFE QUALITY
Land: Austria

An experimental animal model to evaluate the human vector, based on a transgenic mice expressing the receptor for the human virus (hAPN) has been obtained by microinjection

Human coronavirus 229E (HCoV-229E) is a serogroup 1 coronavirus and specific to humans. So far no animal model is available to study the pathogenesis of infection by HCoV-229E. We show here that the expression of aminopeptidase N (APN, also termed CD13), the receptor for HCoV-229E, is required but not sufficient to confer susceptibility in vivo. HCoV-229E infection was facilitated by crossing APN transgenic mice into Stat1-null mice and by adaptation of HCoV-229E to grow in primary Stat1-null fibroblasts. Double transgenic mice allow for the first time the study of human coronavirus group 1 infections in an animal model, in particular viral tropism, replication, recombination and spread in an immunocompromised situation. Furthermore, these mice provide an important tool for the evaluation of biosafety and efficacy of coronavirus-based vectors.

Reported by

University of Vienna
Institute of Animal Breeding and Genetics
1210 Vienna
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