Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

FP5

BIOSAFE-VACCINE-VECT Informe resumido

Project ID: QLK2-CT-2001-00874
Financiado con arreglo a: FP5-LIFE QUALITY
País: Austria

An experimental animal model to evaluate the human vector, based on a transgenic mice expressing the receptor for the human virus (hAPN) has been obtained by microinjection

Human coronavirus 229E (HCoV-229E) is a serogroup 1 coronavirus and specific to humans. So far no animal model is available to study the pathogenesis of infection by HCoV-229E. We show here that the expression of aminopeptidase N (APN, also termed CD13), the receptor for HCoV-229E, is required but not sufficient to confer susceptibility in vivo. HCoV-229E infection was facilitated by crossing APN transgenic mice into Stat1-null mice and by adaptation of HCoV-229E to grow in primary Stat1-null fibroblasts. Double transgenic mice allow for the first time the study of human coronavirus group 1 infections in an animal model, in particular viral tropism, replication, recombination and spread in an immunocompromised situation. Furthermore, these mice provide an important tool for the evaluation of biosafety and efficacy of coronavirus-based vectors.

Contacto

Mathias MÜLLER, (Chair of Molecular Genetics and Biotechnology in Veterinary )
Tel.: +43-1-250775620
Fax: +43-1-250775690
Correo electrónico
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