Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

Validated PCR assays for the diagnosis of Enteromyxum leei in sparids and other fish

The PCR assays for the diagnosis of Enteromyxum leei in gilthead se bream were developed and validated. High sensitivity and specificity were obtained for both lethal (L-PCR) and non-lethal (NL-PCR) tests. The superior performance of PCR assays respect to gold standard diagnosis is remarkable, especially for L-PCR on posterior intestine and NL-PCR.

Considering these results, NL-PCR is recommended as routine diagnostic method to monitor the status of fish for enteromyxosis. The validation studies evidenced the difficulties of obtaining an accurate diagnosis using routine histology, which enhances the usefulness of PCR tests.

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Reported by

Consejo Superior de Investigaciones Científicas (CSIC)
Instituto de Acuicultura Torre de la Sal , Ribera de Cabanes
12595 Castellón
Spain
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