Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

Method of inducing acute bladder instabilities in an animal model by means of alpha-chloralose

Within the REBEC project it was possible to develop two animal models representing almost the same conditions of bladder dysfunction as in humans with an unstable bladder for the first time. In the acute cystitis model it has been possible to induce bladder instabilities with a fast onset in the Göttinger minipig model as well as in the rabbit model by means of formalin instillations into the bladder. Therefore a special method of anaesthesia had to be developed as normal anaesthesia suppresses involuntary bladder contractions. In the chronic cystitis model within rats, mustard oil was used to induce a chronic inflammation of the bladder without the need for this special anaesthesia.

The acute models provide a fast onset of involuntary bladder contractions within several minutes to one hour with a duration time of up to 5 hours. At this, the concentration of formalin instilled into the bladder can induce hyperactive bladder syndrome without acute inflammation process (like interstitial cystitis) or, using higher concentration, induce symptoms of acute cystitis with inflammation signs and concomitant bladder instabilities. The chronic cystits model within rats provides a much slower onset of involuntary bladder contractions after 24hours and duration for several days. The chronic cystitis model therefore allows investigations on awake rats under normal life conditions if needed.

These two animal models allow scientific investigations under almost the same inflammatory and pathophysiological conditions of overactive bladder syndrome as in humans. Pharmacological investigations as well as investigations on neuromodulation effects and scientific investigations on the neurophysiological and neuroanatomical conditions due to overactive bladder conditions can thus be conducted. These models therefore provide the basis for scientific research on all kinds of treatment methods for overactive bladder in a reliable animal model.

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Klaus-Peter JÜNEMANN, (Head of Department)
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