Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

In vivo model for testing matrix molecules in bone healing

Animal models for bone formation are laborious. Examples in rodents include drilling a critical size defect in the skull bone. The present project has shown that another critical size defect method in rats can give consistent and repeatable results.

A cylindrical defect with a diameter of 2.3 mm is drilled in the mandibular bone. The defect is filled with a carrier, such as equine collagen sponge soaked with the substance to be tested or phosphate buffered saline (PBS) as control. The lower jaws are collected 1-3 weeks after surgery, when the animals are sacrificed. The jaws are then prepared for histological examination. The histomorphometric analyses can indicate the amounts or concentrations needed for statistically significant bone formation. It can also show biocompatibility of formulations, degradation time as well as inflammatory and/or cellular reactions.

The defect used in this model imitates bony defects with remaining bone walls surrounding or bordering the defect, such as those that can be observed after tooth extraction or tooth loss due to periodontitis or failed endodontic treatment.

Reported by

University of Ulm
Albert-Einstein-Allee 11
89070 Ulm
Germany
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