Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

FP5

CRYMCEPT Résumé de rapport

Project ID: QLK5-CT-2002-01279
Financé au titre de: FP5-LIFE QUALITY
Pays: France

The study of the thermal behaviour of water using differential scanning calorimetry (DSC) analysis is a powerful tool to optimise plant cryopreservation protocols

The study of the thermal behaviour of water using Differential Scanning Calorimetry (DSC) analysis is a powerful tool to optimise plant cryopreservation protocols based on osmotic or evaporative dehydration before cooling. DSC can be used to predict the optimal:
- Time of exposure to the Plant Vitrification Solution 2 (PVS2) (vitrification technique)
- Duration of desiccation over silica-gel (encapsulation-dehydration technique)
- Period of drying in the laminar air flow of a sterile cabinet (encapsulation-dehydration technique)
- Optimal water content for non-orthodox oily seed cryopreservation (equilibrium drying technique).

In all cases, DSC is employed to ascertain how to avoid/minimize the formation of lethal intracellular ice crystals in the plant material to be cryopreserved. The study of the thermal behaviour of water could therefore be a valuable way to reduce the number and/or size of trial-and-error tests usually performed to set up a cryopreservation protocol for any new given accession. Since protocols based on the osmotic or evaporative dehydration of the plant material before cooling represent to date the most employed procedures for plant genetic resources cryopreservation, by reducing the time required for protocol optimisation, Result 1 should allow to speed up the introduction of endangered genetic resources in European plant germplasm cryobanks.

Informations connexes

Contact

Stéphane DUSSERT, (Scientist)
Tél.: +33-4-67416185
Fax: +33-4-67416330
E-mail