Servizio Comunitario di Informazione in materia di Ricerca e Sviluppo - CORDIS

Protocol for the transient, dsRNA based silencing of putative pathogenicity factors from cyst nematodes

RNA interference (RNAi) has been used widely as a tool for examining gene function and a method that allows its use with plant-parasitic nematodes recently has been described.

Here, we use a modified method to analyze the function of secreted â-1,4, endoglucanases of the potato cyst nematode Globodera rostochiensis, the first in vivo functional analysis of a pathogenicity protein of a plantparasitic nematode. Knockout of the â-1,4, endoglucanases reduced the ability of the nematodes to invade roots. We also use RNAi to show that gr-ams-1, a secreted protein of the main sense organs (the amphids), is essential for host location.


John HILLMAN, (Head of Institute)
Tel.: +44-1382-562731
Fax: +44-1382-562426