Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

FP5

NONEMA Berichtzusammenfassung

Project ID: QLK5-CT-1999-01501
Gefördert unter: FP5-LIFE QUALITY
Land: Belgium

Discovery of ubiquitin extension proteins as pathogenicity factors involved in feeding site induction

By performing cDNA AFLP on pre- and early parasitic juveniles, we identified genes encoding a novel type of ubiquitin extension proteins secreted by the dorsal pharyngeal gland in the cyst nematode Heterodera schachtii. The proteins consist of three domains, a signal peptide for secretion, a mono-ubiquitin domain, and a short C-terminal positively charged domain. A gfp-fusion of this protein is targeted to the nucleolus in tobacco BY-2 cells. We hypothesize that the C-terminal peptide might have a regulatory function during syncytium formation in plant roots.

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