Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

List and informativeness of markers used in the Churra population

Main results in this point refers to multiplex combination used to genome scan for QTL detection in Churra sheep. For building multiplex reactions from 2 to 4 microsatellites were amplified simultaneously using 4-colours-one lane technologies using an ABI-Prism 377 automatic sequencer. Optimisation of multiplex-PCR reaction involved several different factors to control. We chose this technique with the aim of maximizing economical and time resources These results are interesting for scientific community and will be published in scientific journals for its diffusion.

Reported by

Dep. Produccion Animal
Universidad de Leon
24071 Leon
Spain
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