Community Research and Development Information Service - CORDIS

Microsatellites for analysis of genetic diversity in P. alba and P. euphratica

We have tested microsatellite loci, already developed on Populus trichocarpa, Populus nigra and Populus tremuloides, and developed RAPD markers to characterize Populus alba ecotypes with different attitude in salt stress tolerance. Using 79 microsatellite loci the segregation in F1 population was detected between the progeny of tolerant and untolerant ecotypes. 54 RAPD markers were able to distinguish tolerant and untolerant ecotypes and their progeny and some of them have been developed as SCAR markers.

The results obtained were 78 microsatellite primer sequences and 30 RAPD primer sequences, and the latter markers 3 potential SCAR markers have been also developed. Moreover, genomic sequences and information on degree of polymorphism in P. alba have also be obtained. Actually both type of markers are using to identify Quantitative Traits Loci related to canopy development and salt tolerance in P. alba and P. euphratica.

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DISAFRI - University of Tuscia
Via S. Camillo de Lellis, snc
01100 VITERBO
Italy
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