Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

A reference panel of map isolate strains from European wildlife and domestic species

A panel of 122 field isolates of Map from the Czech Republic (24), The Netherlands (38), Scotland (46) and Spain (14) was assembled. The isolates were from both domestic livestock and wildlife as follows: cow (Bos taurus)(39), sheep (Ovis aries) (11), goat (Capra hircus) (17), moufflon (Ovis musimon) (7), red deer (Cervus elaphus) (11), fallow deer (Dama dama) (4), badger (Meles meles) (1), fox (Vulpes vulpes) (3), stoat (Mustela erminea) (4), weasel (Mustela nivalis) (2), crow (Corvus corone)(1), rook (Corvus frugilegus) (1), jackdaw (Corvus monedula)(1), rabbit (Oryctolagus cuniculus) (14), hare (Lepus europaeus) (2), rat (Rattus norvegicaus) (1), wood mouse (Apodemus sylvaticus) (1), giraffe (Giraffa camelopardalis) (1) and cat (Felis domesticus) (1). The panel of isolates was typed by amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism and hybridization to IS900 (IS900-RFLP) and pulsed-field gel electrophoresis (PFGE)although not all isolates were typed with all methods due to problems incurred when subculturing. The panel provides a valuable resource for the scientific community for further studies on paratuberculosis, particularly those relating to strain differentiation and epidemiology. No funds exist to maintain stocks of the panel but the individual partners may have DNA stocks available for further studies.

Reported by

MOREDUN RESEARCH INSTITUTE
Pentlands Science Park, Bush Loan -
PENICUIK
United Kingdom
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