Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

SSLP based procedure to detect rare apomicts in a sexual population

To screen for putative apomictic embryos possibly due to introduction of a key gene into a fully functional sexual background the Single Sequence Length Polymorphism (SSLP) assay was used to detect plants heterozygous for up to 15 markers. F2 populations of AtDMC1: AtSERK1 overexpressing plants generated during year 1 and 2 were screened for putative apomictic seeds using the SSLP analysis. The population showed segregation pattern similar to the wt. The highest number of heterozygous SSLP markers in a single plant found in this population was 15 and the chance to get this plant by chance only was more then 1/4000. Thus the procedure is sensitive enough to find as low is 0.1 % of potential apomicts within an otherwise sexually propagated population of plants.

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