Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

FP5

EASYRING Berichtzusammenfassung

Project ID: QLK4-CT-2002-02286
Gefördert unter: FP5-LIFE QUALITY
Land: Germany

Establishment of retinol binding protein, transthyretin and transferrin as "new" biomarkers for ED

Endocrine disrupting chemicals (EDC) are known to interfere with the normal endocrine system of different animals including humans. EDC can have adverse effects on the reproduction like changed sex ratios, intersex phenomena, reproductive malformations or reduced fecundity.

The use of mRNA expression of retinol binding protein (RBP), transthyretin (TTR) and transferrin (TF) were investigated as potential biomarkers for EDC in the liver of Xenopus laevis as a model organism. For this reason, X. laevis were exposed in vivo to an estrogenic compound, ethinylestradiol (EE2), an antiestrogenic compound, tamoxifen (TAM), an androgenic compound, methyldihydrotestosterone (MDHT), an antiandrogenic compound, flutamide (FLU) at a concentration of 10-8M and to Lambro river water for four weeks. TTR mRNA expression was inhibited by estrogens in male and female frogs.

In contrary, RBP mRNA was up-regulated by estrogens in males and females. The mRNA expression of TF was down-regulated by estrogens in males and females but also upregulated by antiestrogens in females.

Summarising RBP and TTR can serve as estrogenic biomarker while TF can be used as (anti)estrogenic biomarker.

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