Servizio Comunitario di Informazione in materia di Ricerca e Sviluppo - CORDIS

Use of sex steroid, biomarker and histology of selected ED in Xenopus. (A joint cooperation between Genova and Berlin)

The impact of endocrine disrupting chemicals (EDC) on sex steroids, biomarkers and histology in Xenopus laevis were investigated. This combined approach of different endpoints for endocrine disruption should deliver information about the sensitivity of examined endpoints for the disruption of normal endocrine function.

For this reason, X. laevis were exposed in vivo to an estrogenic compound, ethinylestradiol (EE2), an antiestrogenic compound, tamoxifen (TAM), an androgenic compound, methyldihydrotestosterone (MDHT), an antiandrogenic compound, flutamide (FLU) at a concentration of 10-8M and to Lambro river water for four weeks. Biomarkers (RBP, TTR and TF as described in detail at Nr. 1 of main results) and sex steroids (estradiol and testosterone) were changed by (anti)estrogenic but not by (anti)androgenic model compounds.

In contrary, severe changes in the histology of gonads of X. laevis were found by all treatments including the exposure to Lambro river water.

Summarising, histology of gonads was a suitable endpoint for endocrine disruption indicating (anti)estrogenic and (anti)androgenic mode of actions. The mRNA expression of examined genes and sex steroids showed endocrine disruption by (anti)estrogenic mode of action. Biomarkers for (anti)androgenic mode of actions have still to be developed.

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