Servizio Comunitario di Informazione in materia di Ricerca e Sviluppo - CORDIS

New methods in GC-MS and LC-MS

Results are concerned with the development and application of new analytical methods in GC-MS and LC-MS/MS for the identification and quantification of selected endocrine disrupting compounds in environmental and biological samples: water, sediment, macroinvertebrates, tissue from fishes plasma, bile, liver, muscle.

The analytical work has been concentrated on a restricted number of potentially effective chemicals confirmed both to be endocrine disrupting substances and, in the mean time, to be really present in relevant way in the areas utilised for in field applicability of novel testing methods. The selected analytes have been estrone, estradiol, ethynilestradiol, estrone-gluoronide, estradiol-3gluronide, estradiol-17glucoronide, octylphenol, nonylphenol mix, bisphenol A, tertoctylphenol.

Experiments have been performed in order to optimise the sample preparation steps, mainly based on solid phase (SPE) methodologies. GC-MS and LC-MS/MS procedures have been developed. Studies have been performed in order to determine the characteristics of the developed procedures in term of reproducibility, limits of detection and quantification and linearity.

Real wild-life exposure and controlled environments has been studied to characterize environmental level of EDCs and to evaluate experimentally the xenobiotic concentrations able to induce the disruptive response in the studied organisms.
Analytical methods will be published and freely available in scientific literature.

Reported by

Mario Negri Pharmacological Research Institute
via Eritrea 62
20157 Milan
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