Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

FP5

ASIARESIST Berichtzusammenfassung

Project ID: ICA4-CT-2001-10028
Gefördert unter: FP5-INCO 2
Land: Belgium

Central collection of chloramphenicol-resistant heterotrophs from aquaculture environments

A central backup collection of all isolates collected in this project was established that is accessible to all partners of the project. The principle of storage is based on cryopreservation using the SOP-ASIARESIST-PRES document. The final strain collection consists of 724 chloramphenicol-resistant (CHLR) isolates (C isolates) and 102 chloramphenicol-susceptible isolates (N isolates).

Upon receipt, all isolates were checked for purity and single colony morphology prior to cryopreservation. A number of cultures could not be recovered or purified upon arrival and were resent by the involved partners. According to colony morphology, a number of isolates displayed the typical morphology of yeasts. This was further checked by microscopic analysis of cell morphology and determination of resistance to the eukaryotic inhibitor cycloheximide (0.02%).

Likewise the isolates that failed to recover after repeated subculturing, isolates that were classified as yeasts, were discarded from the strain collection. At regular intervals, subsets of checked isolates were sent to University of Stirling (UK) for strain typing. After the end of the project, a representative set of C isolates will be deposited in the public BCCM/LMG Bacteria Collection (http://bccm.belspo.be/db/bacteria_search.htm).

Reported by

GHENT UNIVERSITY
K.L. Ledeganckstraat 35
9000 GENT
Belgium
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