Community Research and Development Information Service - CORDIS

FP5

ASIARESIST Report Summary

Project ID: ICA4-CT-2001-10028
Funded under: FP5-INCO 2
Country: United Kingdom

Plasmid extraction protocol

A mini-prep plasmid extraction protocol was optimised for varied genera encountered within the aquaculture environment based on the alkaline lysis method. The modifications allowed for the extraction of high molecular weight plasmid DNA (> 100 kb) and avoided the use of commercial purification columns and hazardous solvents.

As such the method is ideal as a cost-effective, simple method to screen for plasmid DNA. Although the protocol was originally designed for plasmid profiling chloramphenicol resistant bacteria from SE Asian aquaculture sites, the method can be adapted for other bacterial groups using different bacterial growth conditions. Troubleshooting notes are incorporated into the method as well as recommended agarose gel electrophoresis conditions for the separation of the plasmid DNA samples.

Related information

Reported by

Institute of Aquaculture
University of Stirling, Pathfoot Building
FK9 4LA Stirling
United Kingdom
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