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ASIARESIST Résumé de rapport

Project ID: ICA4-CT-2001-10028
Financé au titre de: FP5-INCO 2
Pays: United Kingdom

Macrorestriction analysis of aquaculture-associated isolates

Pulsed-field gel electrophoresis of macrorestriction fragments was applied to confirm the clonal relationships of aquaculture-associated genera previously inferred by (GTG)5-PCR typing. Cell lysis, restriction enzymes and electrophoresis running conditions were optimised for each species tested.

The majority of isolates yielded suitable profiles for macrorestriction analysis; however, a proportion of the isolates could not be processed due to DNA degradation. Numerical analysis of the resulting pulsotypes, revealed significant agreement between the clonal strain types identified by (GTG)5-PCR typing and macrorestriction analysis.

This confirmed the presence of replicates (identical strains occurring within the same sample) and was able to track strains within the aquaculture environment (clonal strain types occurring between different samples) over time, between distinct niches (water, sediment and cultured organism), ponds and even between farm sites. Macrorestriction analysis was found to be a highly discriminatory genotyping method, in detecting the clonal variation present within a strain collection.

Reported by

University of Stirling
Institute of Aquaculture, Pathfoot Building, University of Stirling
FK9 4LA Stirling
United Kingdom
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