Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

FP5

ASIARESIST Informe resumido

Project ID: ICA4-CT-2001-10028
Financiado con arreglo a: FP5-INCO 2
País: United Kingdom

Gel-Compar database containing descriptive and experimental typing data

A Gel-Compar-based database (Applied Maths, Belgium) was set up to contain descriptive data of the strains in the ASIARESIST strain collection as well as the experimental identification and typing data. These descriptive data include the full strain code of each isolate, the shortened strain reference code and colonial morphology of each isolate.

In the course of the project, experimental data were added to the database from the identification and typing work packages. All ASIARESIST isolates received in Stirling (n= 650) were entered into a strain collection maintained within the Bacteriology Department of the Institute of Aquaculture and processed by (GTG)5-PCR mediating typing. A sub-set of isolates that appeared indistinguishable or highly similar by (GTG)5-PCR typing were also selected for macrorestriction analysis.

The Gel-Compar database contains the stored DNA patterns of these two typing techniques as well as plasmid profiles of selected Vietnam isolates. The database allows individual strains to be compared based on the combined DNA fingerprinting attributes and was applied to de-replicate the strain collection and track strains within the aquaculture environment.

Reported by

University of Stirling
Institute of Aquaculture, Pathfoot Building, University of Stirling
FK9 4LA Stirling
United Kingdom
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