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Frozen pools of yeast transformants of a hypersensitive strain expressing wheat cDNAs

We have generated about 10 million yeast transformants containing plasmids leading to the expression of wheat cDNAs under the strong PGK1 promoter.

The yeast transformants were washed off from nonselective transformation plates and stored at -70°.

Aliquots could be thawed and used to select for resistance conferring plasmids to any substance toxic to the yeast host strain (pdr5,10,15 ayt1, rps11). This should save time end expenses for potential patners needed to generate the library and transforming yeast.

Reported by

Institute of Applied Genetics and Cell Biology,
Muthgasse 18
1190 WIEN
Austria
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